restriction mapping meaning in Chinese
限制制图
Examples
- The restriction map of phzl318 carrying the entire add gene cluster from s . avermitilis nrrl8165 was made . its two subclones phz2104 and phz2105 were introduced into s . lividans mutant zx1
制作了携带完整add基因簇的phz1318的限制内切酶图谱，根据酶谱进行亚克隆得到phz2104和phz2105 。 - By comparing restriction maps of plasmids possessed in these clones , 5 clones ( clones 1 , 4 , 5 , 6 , and 8 ) were found to contain the same chitinase gene , while the other three clones ( clones 2 , 3 and 9 ) contain different chitinase genes one another
经底物反应和限制性内切酶图谱分析，确定其中的clone - 1 ， 4 ， 5 ， 6 ， 8含有相同的几丁质酶基因；而clone - 2 ， 3 ， 5 ， 9四株重组菌则含有不同的几丁质酶基因。 - The restriction map of the plasmid pbl29 was tentatively constructed according as the molecular weights of fragments of plasmid pbl29 digested with different enzymes . analysing the sequence of the plasmid pbl29 tested , we found that the mol ecular weight of the plasmid pbl29 is 371 lbp , that it s many unique restriction endonucleases , km resistance genes and abundant a / t base pairs of replicating site are on the plasmid pbl29
同时根据限制性酶切各片段的分子量作出了质粒pbl29的内切酶图谱。并对质粒pbl29进行测序和分析，证明了质粒pbl29大小为3711bp ，具有大量的单酶切位点、编码卡那霉素抗性基因以及富含a t序列的复制起点。 - Then , the plasmid was transformed into jm109 . the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence . test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program
利用rt - pgr技术，设计一对引物，对田间采集的经鉴定含pstvd的阳性样品进行全序列扩增，并将所得产物纯化回收，连接到pmd18t - vector中，并转化至大肠杆菌jm109中，挑选白斑进行双酶切( saci / ecorv )鉴定证明插入片段为359bp大小，进行序列测定，所得克隆基因为pstvd全序列负链，大小为359bp 。